Novak Djokovic crashed out of Wimbledon on Saturday after going down to Sam Querrrey in four sets.
The World No.1 was beaten 6-7 1-6 6-3 6-7 in a pulsating rain-delayed clash on Court 1.
Djokovic became the first man to win four consecutive grand slams since 1969 when he won last month’s French Open.
It’s the first time the Serb has suffered defeat at at a grand slam since the 2015 French Open final.
Querrey, seeded 28th and ranked 41 in the world, had led by two sets to none when rain forced play to be suspended on Friday.
Djokovic fought back to take the third but the American held his nerve to win the fourth on a tiebreak and made history.
“It’s incredible, especially to do it here at Wimbledon,” Querrey told the BBC.
“I’m so ecstatic, so happy. I think today I played the break points well.
“Every time he had a break point I was able to come up with a big serve.
“He came out and got the first four games, fortunately for me we had another rain delay and I got to regroup.
“I’m just taking it one round at a time, I’m not even sure who I’m playing next.”
Querrey will face Frenchman Nicolas Mahut for a place in the quarterfinals.